Cell Signaling Technology

PathScan® 4-Plex Array Kits

New PathScan® 4-Plex Array Kits from Cell Signaling Technology® (CST) provide all reagents necessary for a multiplex assay based upon the sandwich ELISA principle. Rather than one immobilized antibody, PathScan 4-Plex Array Kits contain four different antibodies per well in a 96-well plate. This allows the researcher to obtain four times more information per well. In addition, the incorporation of multiple phospho-specific antibody pairs allows the researcher to measure the activation status of important signaling nodes. These kits can be a useful tool for the profiling of chemical compounds.

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  • Antibodies from CST combined with array technology from Quansys Biosciences ensure the highest quality products.
  • Validated to ensure no cross-reactivity among markers within an array.
  • Use only 50 μl of sample to generate greater amounts of data compared to traditional ELISA testing.
  • Can be run on a number of different chemiluminescent imaging systems.
  • Technical support provided by CST Scientists.

Necessary Equipment

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Quansys imager (Cat # 104450GR) and Q-View Plus™ software (Quansys Biosciences, UT; www.quansysbio.com) are recommended for this assay kit for acquisition of the luminescent signal and quantification of spot intensity, respectively. Alternative imagers compatible with this array product are: Alpha Innotech Fluorchem SP; Bio-Rad Versa Doc 4000; Fujifilm LAS-3000; Kodak 4000 MM; UVP EC3 Biochemi.

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