ChIP

• Protocols

The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell. Cell Signaling Technology^® (CST) now has extended its expertise in antibody validation and is providing you with antibodies, kits and reagents for chromatin immunoprecipitation (ChIP).

CST’s new ChIP recommended antibodies have been validated in house by our antibody development scientists by the same rigorous standards as all other recommended applications. Technical support will be provided by the same scientists that validated the antibody, which will guarantee a fast, thorough and accurate response.

CST’s new SimpleChIP™ Enzymatic Chromatin IP Kits are co-developed by CST and NEB scientists and therefore contain the highest quality research reagents. These kits are available with either Protein G agarose or Protein G magnetic beads and contain all buffers and reagents needed to perform up to 30 ChIP assays. The same reagents in these kits have been used for in-house ChIP validation at CST, which will simplify your optimization. The kits can be utilized with any ChIP-validated antibody to detect endogenous levels of protein-DNA interactions and histone modifications in mammalian cells. Protein G agarose and Protein G magnetic beads are also available as separate products.

ChIP Validated Antibodies

No.	Name	Applications	Reactivity
9562	β-Catenin Antibody	W, IP, IHC-P, ChIP	H, M, R, Mk, Z
9581	β-Catenin Antibody (Amino-terminal Antigen)	W, IP, IF-F, F, ChIP	H, M, R, Mk, Hm, B
9587	β-Catenin Antibody (Carboxy-terminal Antigen)	W, IP, IHC-P, IHC-F, IF-F, F, ChIP	H, M, Mk, Mi, Hm, B, (Pg, C, B, X, Dg)
3417	CTCF (D1A7) Rabbit mAb	W, IP, IF-IC, ChIP	H, R, Mk, (B)
3418	CTCF (D31H2) Rabbit mAb	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk, (B)
2899	CTCF Antibody	W, IP, IF-IC, ChIP	H, M, R, Mk
9725	Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk
9726	Di-Methyl-Histone H3 (Lys4) Antibody	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk, (All)
9751	Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk, (All)
9733	Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk, (All)
9756	Tri-Methyl-Histone H3 (Lys27) Antibody	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk, (All)
9649	Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb	W, IP, IHC-P, IF-IC, ChIP	H, M, Z, (R, Mk)
9677	Acetyl-Histone H3 (Lys9/Lys14) Antibody	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk, Z, (All)
9675	Acetyl-Histone H3 (Lys18) Antibody	W, IHC-P, ChIP	H, M, R
9753	Di-Methyl-Histone H3 (Lys9) Antibody	W, IP, IHC-P, IF-IC, ChIP	H, M, R, Mk, (All)
4069	Pan-Methyl-Histone H3 (Lys9) Antibody	W, IP, IF-IC, ChIP	H, M, R, Mk, Z, (All)
2650	Histone H3 Antibody (ChIP Formulated)	ChIP	H, M, (R, Mk, C, B, Dm, X, Z)
2960	Histone H4 (L64C1) Mouse mAb (ChIP Formulated)	ChIP	H, (M, R, Mk, B, Dm, X, Z)
9441	Acetylated-Lysine Antibody	W, IP, IHC-P, IF-IC, ChIP, E-P	All
2729	Normal Rabbit IgG	ChIP
2629	Rpb1 CTD (4H8) Mouse mAb	W, IP, ChIP	H, M, R, Mk, (Sc, Hm, Dm)
9167	Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb	W, IP, IHC-P, IHC-F, F, ChIP	H, M
9171	Phospho-Stat1 (Tyr701) Antibody	W, IP, IF-IC, F, ChIP	H, M, R, (B)
9177	Phospho-Stat1 (Ser727) Antibody	W, IP, IF-IC, ChIP	H, M, R
9172	Stat1 Antibody	W, IP, ChIP	H, M, R, Mk, (B)
9145	Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb	W, IP, IHC-P, IHC-F, IF-IC, F, ChIP	H, M, R
9131	Phospho-Stat3 (Tyr705) Antibody	W, IP, IF-IC, ChIP	H, M, R, C, (B)
9134	Phospho-Stat3 (Ser727) Antibody	W, IP, IF-IC, ChIP	H, M, R, (B)
9132	Stat3 Antibody	W, IP, IHC-P, ChIP	H, M, R, (B)
9139	Stat3 (124H6) Mouse mAb	W, IP, IHC-P, IF-IC, F, ChIP	H, M, R, Mk
3737	SUZ12 (D39F6) Rabbit mAb	W, IP, IF-IC, ChIP	H, M, R, Mk
2569	TCF4 (C48H11) Rabbit mAb	W, IP, ChIP	H, (M, R, C)
2565	TCF4 (C9B9) Rabbit mAb	W, IP, ChIP	H, (M, R)
2566	TCF4 Antibody	W, IP, ChIP	H, (M, C)